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Home > Platforms > Mapping
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Mapping
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FISH paints of human chromosomes 2.
Present day clone based physical map construction is based on the pioneering work that produced physical maps for yeast and C. elegans. Automation has been utilized to create a high throughput process that collects data from clone restriction digests or fingerprints from the cloning vector of choice, bacterial artificial chromosomes (BACs). This process starts with high quality BAC libraries and proceeds through DNA isolation, restriction enzyme digestion and high resolution agarose gel separation. After fluorescent staining and imaging, the data are analyzed using a series of software tools, such as IMAGE, BandLeader and FPC. Other map related software programs are then utilized by a group of experts designated as pathfinders to construct comprehensive physical maps for community use. With the increasing emphasis on whole genome shotgun assemblies, integration of mapping data throughout the assembly and editing process is key to assuring accuracy of the final product. In large genome sequence assemblies to date, mapping data have provided the framework for organizing the sequence. In addition, from these mapsa clone based tile path of the genome can be generated for functional genomic experiments. Furthermore, when a species genome is designated for further sequence characterization these maps serve as a critical resource for efficient gap closure and clone selection.
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| Mapping Contact |
Tina Graves
Group Leader of Mapping
Send email
Washington University School of Medicine
The Genome Center
4444 Forest Park Ave
St. Louis, Missouri 63108
USA
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| Selected Mapping Publications
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Title
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Probabilistic assessment of clone overlapsin DNA fingerprint mapping via a priori models.
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Authors
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Wendl MC. |
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J Comput Biol. 2005 Apr;12(3):283-97. |
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Title
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A physical map of the chicken genome.
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Authors
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Wallis JW, Aerts J, Groenen MA, Crooijmans RP, Layman D, Graves TA, Scheer DE, Kremitzki C, Fedele MJ, Mudd NK, Cardenas M, Higginbotham J, Carter J, McGrane R, Gaige T, Mead K, Walker J, Albracht D, Davito J, Yang SP, ... |
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Nature. 2004 Dec 9;432(7018):679-80. |
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Title
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Fosmid-based physical mapping of the Histoplasma capsulatum genome.
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Authors
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Magrini V, Warren WC, Wallis J, Goldman WE, Xu J, Mardis ER, McPherson JD. |
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Genome Res. 2004 Aug;14(8):1603-9. |
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Title
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Advances in genome biology and technology.
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Authors
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Mardis ER. |
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Pharmacogenomics. 2004 Jun;5(4):355-6. |
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Title
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Integrated and sequence-ordered BAC- and YAC-based physical maps for the rat genome.
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Authors
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Krzywinski M, Wallis J, Gosele C, Bosdet I, Chiu R, Graves T, Hummel O, Layman D, Mathewson C, Wye N, Zhu B, Albracht D, Asano J, Barber S, Brown-John M, Chan S, Chand S, Cloutier A, Davito J, Fjell C, ... |
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Genome Res. 2004 Apr;14(4):766-79. |
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